Characteristics of cells present in peritoneal fluids of mice injected intraperitoneally with Bordetella pertussis

Characteristics of cells present in peritoneal fluids of mice injected intraperitoneally with Bordetella pertussis.

Peritoneal fluids obtained from mice after the intraperitoneal administration of Bordetella pertussis vaccine, heated vaccine, an extract of the organisms, killed Escherichia coli, or thioglycolate medium were examined in terms of total cells and percentage that adhered to glass cover slips during 2-h incubation period. All these substances were found to increase the number of leukocytes in per...

Survival of Bordetella pertussis in Tissue Culture of Animal Cells

IgM antibody production in mice intraperitoneally injected with zirconium oxychloride.

The effect of zirconium (Zr) on the humoral immune response was studied by measuring the level of IgM-plaque forming cells (IgM-PFC) against sheep red blood cells (SRBC) in the spleen of C57 BL mice intraperitoneally injected with zirconium oxychloride. Two experiments, a single injection of zirconium oxychloride of 1/5, 1/10, 1/50, and 1/100 of the LD50 for intraperitoneal injection and contin...

Regulation of pertussis toxin and lipopolysaccharide levels of Bordetella pertussis 134 in response to modulators

  Whooping cough (pertussis) is a highly contagious disease of the human respiratory tract, which is caused by Bordetella pertussis. Reemerge of pertussis in some highly immunized populations and divergency in gene order among several B. pertussis strains promoted this research to study the change of pertussis toxin (PT) and lipopolysacharide levels in response to the different environments. Th...

Regional differences in the growth of tumor cells injected intraperitoneally into syngeneic adult mice.

C755 mammary carcinoma cells were injected i.p. into adult mice of the strain of origin (C57BL/6). By using a common insertion point but directing the needle caudad or craniad, a differential delivery of tumor cells was deliberately achieved. At high cell doses, the median survival time was shorter for animals receiving anterior injections than for those given pos terior injections. At limiting...